Protocols

RNA Isolation and Gel Verification

RNA Isolation Using Trizol and PLG-heavy Tubes
RNA Isolation (Vodkin Method)
RNA Isolation (Damla Bilgin Method)
Quality checking by running gel on Agilent BioAnalyzer


Misc. Lab Protocols

Reverse Transcription of RNA (includes DNAse I and RT protocols)
Gateway Cloning Protocol
pBIN-T Cloning Protocol
PCR Protocol using NEB Taq DNA Polymerase with Standard Taq Buffer (M0273)
LB Agar Plates
High molecular weight DNA extraction
Cloning a 4kb transcript in soybean


Sequencing and Gene Synthesis Order Forms

DNA Sequencing
GeneArt Gene Synthesis


Lab Forms and Signs

PCR Signup Sheet
Biosafety Cabinet Signup Sheet
Clough Lab Cloning Worksheet
Clough Lab Cone Database Template


Zymo Research Protocols

ZR Plasmid Miniprep Classic Kit
ZR Plasmid Miniprep 5ml Modifications
DNA Clean & Concentrator-25 Kit (PCR Cleanup)
Zymoclean Gel DNA Recovery Kit


Qiagen Protocols

(Note: we have switched to Zymo Research kits for most of these)
DNeasy Plant Handbook
QIAprep Miniprep Handbook>
QIAquick PCR Purification Handbook
RNeasy Mini Handbook


Transformation

Arabidopsis - Floral Dip Protocol
NEB Turbo Competent E. coli (High Efficiency)

Agrobacterium protocols

Agrobacterium Plasmid Prep
Agrobacterium Ti Plasmid Prep + PEG 6000 Precipitation
Electroporation Competent Cells
Heat Shock Transformation of Agrobacterium
Electroporation
Triparental Matings


Microarray Protocols

Indirect Labelling with Amino Allyl dUTP, Dec 2009
5X dNTP mix Nov 6, 2009
Direct Labelling RNA
Direct Labelling DNA
Blocking Slides SDS washing


Tissue Culture Protocols

GUS staining
Seed Sterilization

Tissue Culture Media

Germination
Germination Medium

Shoots
Shoot Induction Medium
Shoot Elongation Medium
Modified Shoot Induction Medium

Rooting
Rooting Medium

Other Media
C5 Medium
D1 Medium
Soybean Rescue Medium
Co-cultivation Medium

Stock Solutions
MS Major Stock
MS Minor Stock
MSIII Iron Stock
B5 Major Stock
B5 Minor Stock
B5 Vitamin Stock