Protocols
RNA Isolation and Gel Verification
RNA Isolation Using Trizol and PLG-heavy Tubes
RNA Isolation (Vodkin Method)
RNA Isolation (Damla Bilgin Method)
Quality checking by running gel on Agilent BioAnalyzer
Misc. Lab Protocols
Reverse Transcription of RNA (includes DNAse I and RT protocols)
Gateway Cloning Protocol
pBIN-T Cloning Protocol
PCR Protocol using NEB Taq DNA Polymerase with Standard Taq Buffer (M0273)
LB Agar Plates
High molecular weight DNA extraction
Cloning a 4kb transcript in soybean
Sequencing and Gene Synthesis Order Forms
DNA Sequencing
GeneArt Gene Synthesis
Lab Forms and Signs
PCR Signup Sheet
Biosafety Cabinet Signup Sheet
Clough Lab Cloning Worksheet
Clough Lab Cone Database Template
Zymo Research Protocols
ZR Plasmid Miniprep Classic Kit
ZR Plasmid Miniprep 5ml Modifications
DNA Clean & Concentrator-25 Kit (PCR Cleanup)
Zymoclean Gel DNA Recovery Kit
Qiagen Protocols
(Note: we have switched to Zymo Research kits for most of these)
DNeasy Plant Handbook
QIAprep Miniprep Handbook>
QIAquick PCR Purification Handbook
RNeasy Mini Handbook
Transformation
Arabidopsis - Floral Dip Protocol
NEB Turbo Competent E. coli (High Efficiency)
Agrobacterium protocols
Agrobacterium Plasmid Prep
Agrobacterium Ti Plasmid Prep + PEG 6000 Precipitation
Electroporation Competent Cells
Heat Shock Transformation of Agrobacterium
Electroporation
Triparental Matings
Microarray Protocols
Indirect Labelling with Amino Allyl dUTP, Dec 2009
5X dNTP mix Nov 6, 2009
Direct Labelling RNA
Direct Labelling DNA
Blocking Slides SDS washing
Tissue Culture Protocols
GUS staining
Seed Sterilization
Tissue Culture Media
Germination
Germination Medium
Shoots
Shoot Induction Medium
Shoot Elongation Medium
Modified Shoot Induction Medium
Rooting
Rooting Medium
Other Media
C5 Medium
D1 Medium
Soybean Rescue Medium
Co-cultivation Medium
Stock Solutions
MS Major Stock
MS Minor Stock
MSIII Iron Stock
B5 Major Stock
B5 Minor Stock
B5 Vitamin Stock